Cadherin 5 antibody (pTyr731)

Catalog No. ABIN7218860

The Rabbit Polyclonal anti-Cadherin 5 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Cadherin 5 in samples from Human and Mouse.

Quick Overview for Cadherin 5 antibody (pTyr731) (ABIN7218860)

Target: Cadherin 5 (CDH5)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cadherin 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cadherin 5 Antibody

Binding Specificity: pTyr731

Purpose: VE-Cadherin (phospho Tyr731) Polyclonal Antibody

Specificity: Phospho-VE-Cadherin (Y731) Polyclonal Antibody detects endogenous levels of VE-Cadherin protein only when phosphorylated at Y731.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human VE-Cadherin Phospho-Tyr731

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Alternative Name: VE-Cadherin

Background: Rabbit Anti-VE-Cadherin (phospho Tyr731) Polyclonal Antibody,CDH5, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD antigen CD144,Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin-5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74 % homology.,Cadherin-5

Molecular Weight: observerd band 130kDa

Gene ID: 1003

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2