CaMKIIalpha/delta antibody (pThr286)

Catalog No. ABIN7225194

This anti-CaMKIIalpha/delta antibody is a Rabbit Polyclonal antibody detecting CaMKIIalpha/delta in IF, WB, IHC (p) and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for CaMKIIalpha/delta antibody (pThr286) (ABIN7225194)

Target: CaMKIIalpha/delta

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CaMKIIalpha/delta antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA

Product Details anti-CaMKIIalpha/delta Antibody

Binding Specificity: pThr286

Purpose: CaMKIIα/δ (phospho Thr286) Polyclonal Antibody

Specificity: Phospho-CaMKIIα/δ (T286) Polyclonal Antibody detects endogenous levels of CaMKIIα/δ protein only when phosphorylated at T286.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human CaMKIIalpha/delta Phospho-Thr286

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CaMKIIalpha/delta

Target: CaMKIIalpha/delta

Background: Rabbit Anti-CaMKIIα/δ (phospho Thr286) Polyclonal Antibody,CAMK2A, CAMKA, KIAA0968, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2D, CAMKD, Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II,The product of CAMK2A (calcium/calmodulin dependent protein kinase II alpha) belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for CAMK2A.,Calcium calmodulin-dependent protein kinase type II subunit alpha delta

Molecular Weight: observerd band 54kDa

Gene ID: 815, 817