CHST8 antibody (AA 310-390)

Catalog No. ABIN7224254

This Rabbit Polyclonal antibody specifically detects CHST8 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CHST8 antibody (AA 310-390) (ABIN7224254)

Target: CHST8 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 8 (CHST8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHST8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CHST8 Antibody

Binding Specificity: AA 310-390

Purpose: GalNAc4ST-1 Polyclonal Antibody

Specificity: GalNAc4ST-1 Polyclonal Antibody detects endogenous levels of GalNAc4ST-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human GalNAc4ST-1 at AA range: 310-390

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CHST8

Target: CHST8 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 8 (CHST8))

Alternative Name: GalNAc4ST-1

Background: Rabbit Anti-GalNAc4ST-1 Polyclonal Antibody,CHST8, Carbohydrate sulfotransferase 8, GalNAc-4-O-sulfotransferase 1, GalNAc-4-ST1, GalNAc4ST-1, N-acetylgalactosamine-4-O-sulfotransferase 1,The protein encoded by CHST8 (carbohydrate sulfotransferase 8) belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for CHST8.,Carbohydrate sulfotransferase 8

Molecular Weight: observerd band 40kDa

Gene ID: 64377

UniProt: Q9H2A9