CROT antibody (pThr290)

Catalog No. ABIN7213318

This Rabbit Polyclonal antibody specifically detects CROT in WB, ELISA, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CROT antibody (pThr290) (ABIN7213318)

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CROT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-CROT Antibody

Binding Specificity: pThr290

Purpose: Cot (phospho Thr290) Polyclonal Antibody

Specificity: Phospho-Cot (T290) Polyclonal Antibody detects endogenous levels of Cot protein only when phosphorylated at T290.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cot Phospho-Thr290

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CROT

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Alternative Name: Cot

Background: Rabbit Anti-Cot (phospho Thr290) Polyclonal Antibody,MAP3K8, COT, ESTF, Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2,MAP3K8 is an oncogene that encodes a member of the serine/threonine protein kinase family. Mitogen-activated protein kinase 8 localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.,Mitogen-activated protein kinase kinase kinase 8

Molecular Weight: observerd band 60kDa

Gene ID: 1326

UniProt: P41279

Pathways: Monocarboxylic Acid Catabolic Process