CBL antibody (pTyr674)

Catalog No. ABIN7225600

The Rabbit Polyclonal anti-CBL antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect CBL in samples from Human, Mouse and Rat.

Quick Overview for CBL antibody (pTyr674) (ABIN7225600)

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CBL Antibody

Binding Specificity: pTyr674

Purpose: Cbl (phospho Tyr674) Polyclonal Antibody

Specificity: Phospho-Cbl (Y674) Polyclonal Antibody detects endogenous levels of Cbl protein only when phosphorylated at Y674.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cbl Phospho-Tyr674

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CBL

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Alternative Name: Cbl

Background: Rabbit Anti-Cbl (phospho Tyr674) Polyclonal Antibody,CBL, CBL2, RNF55, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, Signal transduction protein CBL,CBL (Cbl proto-oncogene) is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. CBL has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in CBL are also the cause of Noonan syndrome-like disorder.,E3 ubiquitin-protein ligase CBL

Molecular Weight: observerd band 120kDa

Gene ID: 867

UniProt: P22681

Pathways: TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals