Caspase 4 p20 (AA 40-120), (Cleaved-Gln81) antibody

Catalog No. ABIN7202048

This Rabbit Polyclonal antibody specifically detects Caspase 4 p20 in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Caspase 4 p20 (AA 40-120), (Cleaved-Gln81) antibody (ABIN7202048)

Target: Caspase 4 p20

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 40-120, Cleaved-Gln81

Purpose: Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody

Specificity: Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-4 p20 protein resulting from cleavage adjacent to Q81.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Caspase-4 p20 at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: Caspase 4 p20

Alternative Name: Caspase-4 p20

Background: Rabbit Anti-Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody,CASP4, ICH2, Caspase-4, CASP-4, ICE(rel)-II, Protease ICH-2, Protease TX,CASP4 encodes caspase 4 that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, CASP4 induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.,Caspase-4

Gene ID: 837

UniProt: P49662