Caspase 5 p20 antibody (Cleaved-Asp121)

Catalog No. ABIN7212444

This Rabbit Polyclonal antibody specifically detects Caspase 5 p20 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Caspase 5 p20 antibody (Cleaved-Asp121) (ABIN7212444)

Target: Caspase 5 p20

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 5 p20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caspase 5 p20 Antibody

Binding Specificity: AA 80-160, Cleaved-Asp121

Purpose: Cleaved-Caspase-5 p20 (D121) Polyclonal Antibody

Specificity: Cleaved-Caspase-5 p20 (D121) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-5 p20 protein resulting from cleavage adjacent to D121.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Caspase-5 p20 at AA range: 80-160

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Caspase 5 p20

Target: Caspase 5 p20

Alternative Name: Caspase-5 p20

Background: Rabbit Anti-Cleaved-Caspase-5 p20 (D121) Polyclonal Antibody,CASP5, ICH3, Caspase-5, CASP-5, ICE(rel)-III, Protease ICH-3, Protease TY,CASP5 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of caspase 5 induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by caspase 5, this process requires Fas-mediated dephosphorylation of Max. The expression of CASP5 is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for CASP5.,Caspase-5

Molecular Weight: observerd band 22kDa

Gene ID: 838

UniProt: P51878