Caspase-8 p18 antibody (Cleaved-Ser217)

Catalog No. ABIN7226638

This Rabbit Polyclonal antibody specifically detects Caspase-8 p18 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Caspase-8 p18 antibody (Cleaved-Ser217) (ABIN7226638)

Target: Caspase-8 p18 (Caspase 8 Subunit P18)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase-8 p18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caspase-8 p18 Antibody

Binding Specificity: AA 170-250, Cleaved-Ser217

Purpose: Rabbit Anti-Cleaved-Caspase-8 p18 (S217) Polyclonal Antibody

Specificity: Cleaved-Caspase-8 p18 (S217) Polyclonal Antibody detects endogenous levels of Cleaved-Caspase-8 p18 (S217) protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Cleaved-Caspase-8 p18 (S217) at AA range: 170-250

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Caspase-8 p18

Target: Caspase-8 p18 (Caspase 8 Subunit P18)

Alternative Name: Caspase-8 p18

Background: CASP8, MCH5, Caspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACHCASP8 (caspase 8) encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Molecular Weight: 18kD,54kD

Gene ID: 841

UniProt: Q14790