beta Catenin antibody (pSer33)

Catalog No. ABIN7219816

The Rabbit Polyclonal anti-beta Catenin antibody has been validated for IF, ELISA and IHC (p). It is suitable to detect beta Catenin in samples from Human, Mouse and Rat.

Quick Overview for beta Catenin antibody (pSer33) (ABIN7219816)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-beta Catenin Antibody

Binding Specificity: pSer33

Purpose: Catenin-β (phospho Ser33) Polyclonal Antibody

Specificity: Phospho-Catenin-β (S33) Polyclonal Antibody detects endogenous levels of Catenin-β protein only when phosphorylated at S33.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Catenin-beta Phospho-Ser33

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: Catenin-beta

Background: Rabbit Anti-Catenin-β (phospho Ser33) Polyclonal Antibody,CTNNB1, CTNNB, OK/SW-cl.35, Catenin beta-1, Beta-catenin,Catenin beta-1 encoded by CTNNB1 is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.,Catenin beta-1

Molecular Weight: observerd band 85kDa

Gene ID: 1499

UniProt: P35222

Pathways: Peptide Hormone Metabolism