Caveolin-1 antibody (pTyr14)

Catalog No. ABIN7212902

The Rabbit Polyclonal anti-Caveolin-1 antibody has been validated for WB and ELISA. It is suitable to detect Caveolin-1 in samples from Human, Rat and Mouse.

Quick Overview for Caveolin-1 antibody (pTyr14) (ABIN7212902)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caveolin-1 Antibody

Binding Specificity: pTyr14

Purpose: Caveolin-1 (phospho Tyr14) Polyclonal Antibody

Specificity: Phospho-Caveolin-1 (Y14) Polyclonal Antibody detects endogenous levels of Caveolin-1 protein only when phosphorylated at Y14.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Caveolin-1 Phospho-Tyr14

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: Caveolin-1

Background: Rabbit Anti-Caveolin-1 (phospho Tyr14) Polyclonal Antibody,CAV1, CAV, Caveolin-1,The scaffolding protein (caveolin 1) encoded by CAV1 is the main component of the caveolae plasma membranes found in most cell types. Caveolin 1 links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. CAV1 is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in CAV1 have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.,Caveolin-1

Molecular Weight: observerd band 20kDa

Gene ID: 857

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals