CD276 antibody (Internal Region)

Catalog No. ABIN7218106

The Rabbit Polyclonal anti-CD276 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect CD276 in samples from Human, Mouse and Rat.

Quick Overview for CD276 antibody (Internal Region) (ABIN7218106)

Target: CD276

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD276 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD276 Antibody

Binding Specificity: Internal Region

Purpose: CD276 Polyclonal Antibody

Specificity: CD276 Polyclonal Antibody detects endogenous levels of CD276 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human CD276

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CD276

Target: CD276

Alternative Name: CD276

Background: Rabbit Anti-CD276 Polyclonal Antibody,CD276, B7H3, PSEC0249, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, B7-H3, Costimulatory molecule, CD276,CD276 antigen encoded by CD276 belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.,CD276 antigen

Molecular Weight: observerd band 57kDa

Gene ID: 80381

UniProt: Q5ZPR3

Pathways: Cancer Immune Checkpoints