CD28 antibody (pTyr218)

Catalog No. ABIN7225964

This Rabbit Polyclonal antibody specifically detects CD28 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for CD28 antibody (pTyr218) (ABIN7225964)

Target: CD28

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD28 Antibody

Binding Specificity: pTyr218

Purpose: CD28 (phospho Tyr218) Polyclonal Antibody

Specificity: Phospho-CD28 (Y218) Polyclonal Antibody detects endogenous levels of CD28 protein only when phosphorylated at Y218.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human CD28 Phospho-Tyr218

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: Rabbit Anti-CD28 (phospho Tyr218) Polyclonal Antibody,CD28, T-cell-specific surface glycoprotein CD28, TP44, CD antigen CD28,The protein encoded by CD28 (CD28 Molecule) is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for CD28.,T-cell-specific surface glycoprotein CD28

Molecular Weight: observerd band 45kDa

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response