CDK12 antibody (AA 50-130)

Catalog No. ABIN7227782

The Rabbit Polyclonal anti-CDK12 antibody has been validated for ELISA and WB. It is suitable to detect CDK12 in samples from Human and Rat.

Quick Overview for CDK12 antibody (AA 50-130) (ABIN7227782)

Target: CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-CDK12 Antibody

Binding Specificity: AA 50-130

Purpose: CDK12 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of CDK12 protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human CDK12 protein at AA range: 50-130

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CDK12

Target: CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))

Alternative Name: CDK12

Background: Rabbit Anti-CDK12 Polyclonal Antibody,Cyclin-dependent kinase 12, Cdc2-related kinase, arginine/serine-rich, CrkRS, Cell division cycle 2-related protein kinase 7, CDC2-related protein kinase 7, Cell division protein kinase 12, hCDK12,CDK12 (Cyclin Dependent Kinase 12) is a Protein Coding gene. Among its related pathways are DNA Damage and Formation of HIV elongation complex in the absence of HIV Tat. Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates Ser-5 in CTD repeats that are already phosphorylated at Ser-7, but can also phosphorylate Ser-2. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.,CDK12

Molecular Weight: observerd band 163kDa

Gene ID: 51755

UniProt: Q9NYV4