CEP97 antibody (AA 550-630)

Catalog No. ABIN7225078

The Rabbit Polyclonal anti-CEP97 antibody is suitable to detect CEP97 in samples from Human and Mouse. It has been validated for WB, ELISA and IHC (p).

Quick Overview for CEP97 antibody (AA 550-630) (ABIN7225078)

Target: CEP97 (Centrosomal Protein 97kDa (CEP97))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEP97 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CEP97 Antibody

Binding Specificity: AA 550-630

Purpose: Cep97 Polyclonal Antibody

Specificity: Cep97 Polyclonal Antibody detects endogenous levels of Cep97 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Cep97 at AA range: 550-630

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CEP97

Target: CEP97 (Centrosomal Protein 97kDa (CEP97))

Alternative Name: Cep97

Background: Rabbit Anti-Cep97 Polyclonal Antibody,CEP97, LRRIQ2, Centrosomal protein of 97 kDa, Cep97, Leucine-rich repeat and IQ domain-containing protein 2,CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4. , providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.,Centrosomal protein of 97 kDa

Molecular Weight: observerd band 97kDa

Gene ID: 79598

UniProt: Q8IW35