Claudin 5 antibody (pTyr217)

Catalog No. ABIN7224716

This Rabbit Polyclonal antibody specifically detects Claudin 5 in ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Claudin 5 antibody (pTyr217) (ABIN7224716)

Target: Claudin 5 (CLDN5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Claudin 5 Antibody

Binding Specificity: pTyr217

Purpose: Claudin-5 (phospho Tyr217) Polyclonal Antibody

Specificity: Phospho-Claudin-5 (Y217) Polyclonal Antibody detects endogenous levels of Claudin-5 protein only when phosphorylated at Y217.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Claudin-5 Phospho-Tyr217

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Claudin 5

Target: Claudin 5 (CLDN5)

Alternative Name: Claudin-5

Background: Rabbit Anti-Claudin-5 (phospho Tyr217) Polyclonal Antibody,CLDN5, AWAL, TMVCF, Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF,CLDN5 (claudin 5) encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in CLDN5 have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for CLDN5.,Claudin-5

Molecular Weight: observerd band 23kDa

Gene ID: 7122

UniProt: O00501

Pathways: Hepatitis C