Cofilin antibody (pSer3)

Catalog No. ABIN7221928

This anti-Cofilin antibody is a Rabbit Polyclonal antibody detecting Cofilin in WB, ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Cofilin antibody (pSer3) (ABIN7221928)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cofilin Antibody

Binding Specificity: pSer3

Purpose: Cofilin (phospho Ser3) Polyclonal Antibody

Specificity: Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cofilin Phospho-Ser3

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: Cofilin

Background: Rabbit Anti-Cofilin (phospho Ser3) Polyclonal Antibody,CFL1, CFL, Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform,Cofilin is ubiquitously expressed in eukaryotic cells where it binds to actin, thereby regulating the rapid cycling of actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous (F) actin by bridging two longitudinally associated actin subunits changing the F-actin filament twist. This process is allowed by the dephosphorylation of cofilin ser-3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates cofilin and renders it unable to bind and depolymerise F-actin.,Cofilin-1

Molecular Weight: observerd band 19kDa

Gene ID: 3716

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events