Complement B + Bb (AA 210-290), (Cleaved-Lys260) antibody

Catalog No. ABIN7224154

This Rabbit Polyclonal antibody specifically detects Complement B + Bb in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for Complement B + Bb (AA 210-290), (Cleaved-Lys260) antibody (ABIN7224154)

Target: Complement B + Bb

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: AA 210-290, Cleaved-Lys260

Purpose: Cleaved-Factor B Bb (K260) Polyclonal Antibody

Specificity: Cleaved-Factor B Bb (K260) Polyclonal Antibody detects endogenous levels of fragment of activated Factor B Bb protein resulting from cleavage adjacent to K260.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Factor B Bb at AA range: 210-290

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: Complement B + Bb

Alternative Name: Factor B Bb

Background: Rabbit Anti-Cleaved-Factor B Bb (K260) Polyclonal Antibody,CFB, BF, BFD, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor B,CFB (complement factor B) encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. CFB localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in CFB are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of CFB is 421 bp from the 5' end of the gene for complement component 2.,Complement factor B

Molecular Weight: observerd band 57+85kDa

Gene ID: 629

UniProt: P00751