Cullin 5 antibody (AA 560-640)

Catalog No. ABIN7228100

This Rabbit Polyclonal antibody specifically detects Cullin 5 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cullin 5 antibody (AA 560-640) (ABIN7228100)

Target: Cullin 5 (CUL5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cullin 5 Antibody

Binding Specificity: AA 560-640

Purpose: CUL5 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of CUL5 protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human CUL5 protein at AA range: 560-640

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cullin 5

Target: Cullin 5 (CUL5)

Alternative Name: CUL5

Background: Rabbit Anti-CUL5 Polyclonal Antibody,Cullin-5, CUL-5, Vasopressin-activated calcium-mobilizing receptor 1, VACM-1,Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS (SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.,CUL5

Molecular Weight: observerd band 85kDa

Gene ID: 8065

UniProt: Q93034

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development