Cyclin D2 antibody (pThr280)

Catalog No. ABIN7225726

The Rabbit Polyclonal anti-Cyclin D2 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Cyclin D2 in samples from Human, Mouse and Rat.

Quick Overview for Cyclin D2 antibody (pThr280) (ABIN7225726)

Target: Cyclin D2 (CCND2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cyclin D2 Antibody

Binding Specificity: pThr280

Purpose: Cyclin D2 (phospho Thr280) Polyclonal Antibody

Specificity: Phospho-Cyclin D2 (T280) Polyclonal Antibody detects endogenous levels of Cyclin D2 protein only when phosphorylated at T280.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cyclin D2 Phospho-Thr280

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cyclin D2

Target: Cyclin D2 (CCND2)

Alternative Name: Cyclin D2

Background: Rabbit Anti-Cyclin D2 (phospho Thr280) Polyclonal Antibody,CCND2, G1/S-specific cyclin-D2,The protein encoded by CCND2 (cyclin D2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of CCND2 was observed in ovarian and testicular tumors. Mutations in CCND2 are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).,cyclin-D2

Molecular Weight: observerd band 40kDa

Gene ID: 894

UniProt: P30279

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases