Cyclin H antibody (pThr315)

Catalog No. ABIN7225768

This Rabbit Polyclonal antibody specifically detects Cyclin H in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cyclin H antibody (pThr315) (ABIN7225768)

Target: Cyclin H (CCNH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin H antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cyclin H Antibody

Binding Specificity: pThr315

Purpose: Cyclin H (phospho Thr315) Polyclonal Antibody

Specificity: Phospho-Cyclin H (T315) Polyclonal Antibody detects endogenous levels of Cyclin H protein only when phosphorylated at T315.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cyclin H Phospho-Thr315

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cyclin H

Target: Cyclin H (CCNH)

Alternative Name: Cyclin H

Background: Rabbit Anti-Cyclin H (phospho Thr315) Polyclonal Antibody,CCNH, Cyclin-H, MO15-associated protein, p34, p37,The protein encoded by CCNH (cyclin H)belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of CCNH is found on chromosome 4. Alternate splicing results in multiple transcript variants.,Cyclin-H

Molecular Weight: observerd band 34kDa

Gene ID: 902

UniProt: P51946

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase