Cytokeratin 14/16 (AA 1-80) antibody

Catalog No. ABIN7222058

The Rabbit Polyclonal anti-Cytokeratin 14/16 antibody (ABIN7222058) specifically detects Cytokeratin 14/16 in ELISA, IF, WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cytokeratin 14/16 (AA 1-80) antibody (ABIN7222058)

Target: Cytokeratin 14/16

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 1-80

Purpose: Cytokeratin 14/16 Polyclonal Antibody

Specificity: Cytokeratin 14/16 Polyclonal Antibody detects endogenous levels of Cytokeratin 14/16 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Cytokeratin 14/16 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: Cytokeratin 14/16

Background: Rabbit Anti-Cytokeratin 14/16 Polyclonal Antibody,KRT14, Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT16, KRT16A, Keratin, type I cytoskeletal 16, Cytokeratin-16, CK-16, Keratin-16, K16,KRT14 encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 Molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.,Keratin type I cytoskeletal 14

Molecular Weight: observerd band 52kDa

Gene ID: 3861, 3868, 3872