DIDO1 antibody (AA 130-210)

Catalog No. ABIN7214568

This Rabbit Polyclonal antibody specifically detects DIDO1 in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for DIDO1 antibody (AA 130-210) (ABIN7214568)

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIDO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DIDO1 Antibody

Binding Specificity: AA 130-210

Purpose: Dio-1 Polyclonal Antibody

Specificity: Dio-1 Polyclonal Antibody detects endogenous levels of Dio-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Dio-1 at AA range: 130-210

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for DIDO1

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Alternative Name: Dio-1

Background: Rabbit Anti-Dio-1 Polyclonal Antibody,DIDO1, C20orf158, DATF1, KIAA0333, Death-inducer obliterator 1, DIO-1, hDido1, Death-associated transcription factor 1, DATF-1,Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for DIDO1, encoding multiple isoforms.,Death-inducer obliterator 1

Molecular Weight: observerd band 244kDa

Gene ID: 11083

UniProt: Q9BTC0

Pathways: Hormone Activity