TdT antibody (AA 350-430)

Catalog No. ABIN7220066

This Rabbit Polyclonal antibody specifically detects TdT in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for TdT antibody (AA 350-430) (ABIN7220066)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TdT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TdT Antibody

Binding Specificity: AA 350-430

Purpose: TdT Polyclonal Antibody

Specificity: TdT Polyclonal Antibody detects endogenous levels of TdT protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human TdT at AA range: 350-430

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: TdT

Background: Rabbit Anti-TdT Polyclonal Antibody,DNTT, TDT, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase,DNTT is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.,DNA nucleotidylexotransferase

Molecular Weight: observerd band 60kDa

Gene ID: 1791

UniProt: P04053

Pathways: DNA Damage Repair