DAB1 antibody (pTyr232)

Catalog No. ABIN7225606

This Rabbit Polyclonal antibody specifically detects DAB1 in WB, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for DAB1 antibody (pTyr232) (ABIN7225606)

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-DAB1 Antibody

Binding Specificity: pTyr232

Purpose: Dab1 (phospho Tyr232) Polyclonal Antibody

Specificity: Phospho-Dab1 (Y232) Polyclonal Antibody detects endogenous levels of Dab1 protein only when phosphorylated at Y232.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Dab1 Phospho-Tyr232

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for DAB1

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Alternative Name: Dab1

Background: Rabbit Anti-Dab1 (phospho Tyr232) Polyclonal Antibody,DAB1, Disabled homolog 1,The Reelin signaling pathway plays a critical role in neuronal development. Reelin is a secreted glycoprotein that binds to the lipoprotein receptors VLDLR and ApoER2 or alpha3beta1 integrin on the surface of neurons. Activation of these receptors induces tyrosine phosphorylation of Disabled 1 (Dab1), an intracellular adaptor. It is generally believed that tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is the most critical downstream event in Reelin signaling. The phosphotyrosine-binding (PTB) domain within its amino terminus enables Dab1 to recognize and bind to a conserved sequence motif within the cytoplasmic tail of the receptors. In addition, the PTB contains a Pleckstrin Homology-like subdomain that binds to phosphoinositides. The phosphoinositide-binding region within the Dab1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab1 independent of VLDLR and ApoER2. It has been demonstrated that Src, CrkII, CrkL and Dock1 associate with tyrosine-phosphorylated Dab. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232.,Disabled homolog 1

Molecular Weight: observerd band 60kDa

Gene ID: 8674

UniProt: O75553