DAB1 antibody (pTyr232)

Catalog No. ABIN684343

Product Details anti-DAB1 Antibody

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Binding Specificity: pTyr232

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This phosphorylation site is found at Tyr232 in Mouse, Rat, and Bovine DAB1.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Dab1 around the phosphorylation site of Tyr232

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DAB1

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Alternative Name: Dab1 (DAB1 Products)

Synonyms: AI956902 antibody, C630028C02Rik antibody, scm antibody, scr antibody, scrambler antibody, yot antibody, disabled-1 antibody, LOC100232823 antibody, si:dkey-242m13.1 antibody, DAB1 antibody, zgc:136547 antibody, DAB1, reelin adaptor protein antibody, disabled 1 antibody, disabled homolog 1 antibody, Dab, reelin signal transducer, homolog 1a (Drosophila) antibody, Dab, reelin signal transducer, homolog 1b (Drosophila) antibody, DAB1 antibody, Dab1 antibody, LOC100232823 antibody, dab1a antibody, dab1b antibody

Background:

Synonyms: Disabled homolog 1, DAB1

Background: Disabled 1 (Dab1) is an 80 kDa protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases. Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 serine 491 is phosphorylated in a Cdk5-dependent manner and regulates, likely indirectly, Reelin-induced signaling during neural cortex development.

Gene ID: 1600

UniProt: O75553