DNA Polymerase I (AA 610-690) antibody

Catalog No. ABIN7219268

This Rabbit Polyclonal antibody specifically detects DNA Polymerase I in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for DNA Polymerase I (AA 610-690) antibody (ABIN7219268)

Target: DNA Polymerase I

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Binding Specificity: AA 610-690

Purpose: DNA pol ι Polyclonal Antibody

Specificity: DNA pol ι Polyclonal Antibody detects endogenous levels of DNA pol ι protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human DNA pol ι at AA range: 610-690

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: DNA Polymerase I

Alternative Name: DNA pol ι

Background: Rabbit Anti-DNA pol ι Polyclonal Antibody,POLI, RAD30B, DNA polymerase iota, Eta2, RAD30 homolog B,POLI (Polymerase (DNA) Iota) is a Protein Coding gene. Among its related pathways are DNA Double-Strand Break Repair and Fanconi anemia pathway. GO annotations related to this gene include damaged DNA binding and DNA-directed DNA polymerase activity. An important paralog of this gene is POLH. rror-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5-deoxyribose phosphate at abasic sites, but may not have lyase activity.,DNA polymerase iota

Molecular Weight: observerd band 85kDa

Gene ID: 11201

UniProt: Q9UNA4