RAD51 antibody (pThr309)

Catalog No. ABIN7223900

This Rabbit Polyclonal antibody specifically detects RAD51 in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for RAD51 antibody (pThr309) (ABIN7223900)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAD51 Antibody

Binding Specificity: pThr309

Purpose: Rad51 (phospho Thr309) Polyclonal Antibody

Specificity: Phospho-Rad51 (T309) Polyclonal Antibody detects endogenous levels of Rad51 protein only when phosphorylated at T309.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Rad51 Phospho-Thr309

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: Rad51

Background: Rabbit Anti-Rad51 (phospho Thr309) Polyclonal Antibody,RAD51, RAD51A, RECA, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A,The protein encoded by RAD51 (RAD51 recombinase) is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for RAD51.,DNA repair protein RAD51 homolog 1

Gene ID: 5888

UniProt: Q06609

Pathways: DNA Damage Repair