DOK3 antibody (AA 70-150)

Catalog No. ABIN7214606

The Rabbit Polyclonal anti-DOK3 antibody is suitable to detect DOK3 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for DOK3 antibody (AA 70-150) (ABIN7214606)

Target: DOK3 (Docking Protein 3 (DOK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DOK3 Antibody

Binding Specificity: AA 70-150

Purpose: Rabbit Anti-Dok-3 Polyclonal Antibody

Specificity: Dok-3 Polyclonal Antibody detects endogenous levels of Dok-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Dok-3 at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DOK3

Target: DOK3 (Docking Protein 3 (DOK3))

Alternative Name: Dok-3

Background: DOK3, Docking protein 3, Downstream of tyrosine kinase 3Dok-1, Dok-2 and Dok-3 are members of a class of "docking" proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Dok-2 (also designated p56 Dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl. Dok-3 is an adapter involved in the recruitment of inhibitory molecules and is highly expressed in B cells and macrophages. Immunoreceptor-mediated cellular activation induces tyrosine phosphorylation of Dok-3. Upon phosphorylation, Dok-3 binds to 5' inositol phosphatase SHIP and the protein tyrosine kinase Csk. Dok-3 may play a significant role in the negative regulation of immunoreceptor signaling in hemopoietic cells.

Molecular Weight: 58kD

Gene ID: 79930

UniProt: Q7L591