DOK2 antibody (pTyr299)

Catalog No. ABIN7212938

The Rabbit Polyclonal anti-DOK2 antibody is suitable to detect DOK2 in samples from Human, Mouse and Monkey. It has been validated for WB, ELISA and IF.

Quick Overview for DOK2 antibody (pTyr299) (ABIN7212938)

Target: DOK2

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-DOK2 Antibody

Binding Specificity: pTyr299

Purpose: Dok-2 (phospho Tyr299) Polyclonal Antibody

Specificity: Phospho-Dok-2 (Y299) Polyclonal Antibody detects endogenous levels of Dok-2 protein only when phosphorylated at Y299.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Dok-2 Phospho-Tyr299

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for DOK2

Target: DOK2

Alternative Name: Dok-2

Background: Rabbit Anti-Dok-2 (phospho Tyr299) Polyclonal Antibody,DOK2, Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2),Docking protein 2 encoded by DOK2 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210 (bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 2 binds p120 (RasGAP) from CML cells.,Docking protein 2

Molecular Weight: observerd band 48kDa

Gene ID: 9046

UniProt: O60496