E2F4/E2F5 (E2F4/5) (AA 30-110) antibody

Catalog No. ABIN7214648

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB, ELISA and IHC (p). Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for E2F4/E2F5 (E2F4/5) (AA 30-110) antibody (ABIN7214648)

Target: E2F4/E2F5 (E2F4/5)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 30-110

Purpose: E2F-4/5 Polyclonal Antibody

Specificity: E2F-4/5 Polyclonal Antibody detects endogenous levels of E2F-4/5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human E2F-4/5 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: E2F4/E2F5 (E2F4/5)

Alternative Name: E2F-4/5

Background: Rabbit Anti-E2F-4/5 Polyclonal Antibody,E2F4, Transcription factor E2F4, E2F-4, E2F5, Transcription factor E2F5, E2F-5,E2F transcription factor 4 encoded by E2F4 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.,Transcription factor E2F4

Molecular Weight: observerd band 44kDa

Gene ID: 1874, 1875

Pathways: Mitotic G1-G1/S Phases