The Mouse Monoclonal anti-EFHD1 antibody has been validated for WB, IF and IHC (p). It is suitable to detect EFHD1 in samples from Human, Mouse and Rat.
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:2000), IF (1:100-1:200), IHC-P (1:50-1:300).
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Target
EFHD1
(EF-Hand Domain Family, Member D1 (EFHD1))
Alternative Name
EFHD1
Background
Mouse Anti-EFHD1 Monoclonal Antibody,EFHD1 encodes a member of the EF-hand super family of calcium binding proteins, which are involved in a variety of cellular processes including mitosis, synaptic transmission, and cytoskeletal rearrangement. EF-hand domain family member D1 encoded by EFHD1 is composed of an N-terminal disordered region, proline-rich elements, two EF-hands, and a C-terminal coiled-coil domain. EF-hand domain family member D1 has been shown to associate with the mitochondrial inner membrane, and in HeLa cells, acts as a novel mitochondrial calcium ion sensor for mitochondrial flash activation. Alternative splicing results in multiple transcript variants.,EF-hand domain-containing protein D1