eIF3zeta (AA 70-150) antibody

Catalog No. ABIN7225596

This Rabbit Polyclonal antibody specifically detects eIF3zeta in ELISA, WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for eIF3zeta (AA 70-150) antibody (ABIN7225596)

Target: eIF3zeta

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 70-150

Purpose: eIF3ζ Polyclonal Antibody

Specificity: EIF3ζ Polyclonal Antibody detects endogenous levels of eIF3ζ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human eIF3zeta at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: eIF3zeta

Background: Rabbit Anti-eIF3ζ Polyclonal Antibody,EIF3D, EIF3S7, Eukaryotic translation initiation factor 3 subunit D, eIF3d, Eukaryotic translation initiation factor 3 subunit 7, eIF-3-zeta, eIF3 p66,Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by EIF3D (eukaryotic translation initiation factor 3 subunit D) is the major RNA binding subunit of the eIF3 complex.,Eukaryotic translation initiation factor 3 subunit D

Molecular Weight: observerd band 64kDa

Gene ID: 8664

UniProt: O15371