Endonuclease G antibody
Quick Overview for Endonuclease G antibody (ABIN7230168)
Target
See all Endonuclease G (ENDOG) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purpose
- NUCG Polyclonal Antibody
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Specificity
- The antibody detects endogenous levels of NUCG protein
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Purification
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen
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Immunogen
- Synthesized peptide derived from part region of human NUCG protein
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Isotype
- IgG
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Application Notes
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).
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Comment
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Primary Antibody
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- Endonuclease G (ENDOG)
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Alternative Name
- NUCG
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Background
- Rabbit Anti-NUCG Polyclonal Antibody,Endonuclease G, mitochondrial, Endo G,Cleaves DNA at double-stranded (DG)n. DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity).,NUCG
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Molecular Weight
- observerd band 32kDa
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Gene ID
- 2021
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UniProt
- Q14249
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Pathways
- Apoptosis
Target
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