EID1 antibody (AA 40-120)

Catalog No. ABIN7214702

This anti-EID1 antibody is a Rabbit Polyclonal antibody detecting EID1 in ELISA, WB, IF and IHC (p). Suitable for Human.

Quick Overview for EID1 antibody (AA 40-120) (ABIN7214702)

Target: EID1 (EP300 Interacting Inhibitor of Differentiation 1 (EID1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EID1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EID1 Antibody

Binding Specificity: AA 40-120

Purpose: EID-1 Polyclonal Antibody

Specificity: EID-1 Polyclonal Antibody detects endogenous levels of EID-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human EID-1 at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EID1

Target: EID1 (EP300 Interacting Inhibitor of Differentiation 1 (EID1))

Alternative Name: EID-1

Background: Rabbit Anti-EID-1 Polyclonal Antibody,EID1, C15orf3, CRI1, RBP21, PNAS-22, PTD014, EP300-interacting inhibitor of differentiation 1, 21 kDa pRb-associated protein, CREBBP/EP300 inhibitory protein 1, E1A-like inhibitor of differentiation 1, EID-1,E1A-like inhibitor of differentiation-1 (EID-1), an acetyltransferase enzyme, binds both the retinoblastoma protein (Rb), a regulator of cell cycle and tissue specific transcription, and the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. EID-1 inhibits cellular differentiation by blocking the histone acetyltransferase activity of p300. EID-1 also acetylates both histones and non-histone proteins such as NCOA3 co-activator. By acetylating histones, EID-1 gives a specific tag for transcriptional activation. In addition to binding Rb and p300, EID-1 also binds to phosphorylated CREB protein, mediating cAMP gene regulation. EID-1 augments the activity of phosphorylated CREB and activates transcription of cAMP responsive genes as a co-activator.,EP300-interacting inhibitor of differentiation 1

Molecular Weight: observerd band 21kDa

Gene ID: 23741

UniProt: Q9Y6B2