EIF2AK1 antibody (AA 540-620)

Catalog No. ABIN7215334

The Rabbit Polyclonal anti-EIF2AK1 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect EIF2AK1 in samples from Human, Mouse and Monkey.

Quick Overview for EIF2AK1 antibody (AA 540-620) (ABIN7215334)

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF2AK1 Antibody

Binding Specificity: AA 540-620

Purpose: HRI Polyclonal Antibody

Specificity: HRI Polyclonal Antibody detects endogenous levels of HRI protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human HRI at AA range: 540-620

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EIF2AK1

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Alternative Name: HRI

Background: Rabbit Anti-HRI Polyclonal Antibody,EIF2AK1, HRI, KIAA1369, Eukaryotic translation initiation factor 2-alpha kinase 1, Heme-controlled repressor, HCR, Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Heme-regulated inhibitor, Hemin-sensitive initiation factor 2,Eukaryotic translation initiation factor 2-alpha kinase 1encoded by EIF2AK1 acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.,Eukaryotic translation initiation factor 2-alpha kinase 1

Molecular Weight: observerd band 71kDa

Gene ID: 27102

UniProt: Q9BQI3

Pathways: Hepatitis C