EIF4A1 antibody

Catalog No. ABIN7203768

The Mouse Monoclonal anti-EIF4A1 antibody has been validated for WB, IF and IHC (p). It is suitable to detect EIF4A1 in samples from Human, Rat and Mouse.

Quick Overview for EIF4A1 antibody (ABIN7203768)

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF4A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF4A1 Antibody

Purpose: eIF4A1 Monoclonal Antibody

Specificity: The antibody detects endogenous eIF4A1 protein.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Immunogen: Synthetic Peptide

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000), IF (1:100-1:200), IHC-P (1:50-1:300).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EIF4A1

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Alternative Name: eIF4A1

Background: Mouse Anti-eIF4A1 Monoclonal Antibody,eIF4A1 is a 406 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family and eukaryotic initiation factor-4A subfamily with a helicase ATP-binding domain and a helicase C-terminal domain. eIF4F eukaryotic translation initiation complex is formed by the association of eIF4A1 with eIF4E and eIF4E kinase (MNK1), eIF4B, eIF4G, PABPC1. It is closely related to eIF4A2. eIF4A1 is a eukaryotic translation initiation factor involved in protein translation/synthesis where it helps in unwinding the secondary structure of RNA in the 5'-UTR of mRNAs, cap recognition, and is necessary for efficient binding mRNA to the 40S small ribosomal subunit, as well as subsequent scanning for the initiator codon. Ubiquitous expression of the enzyme eIF4A1 is seen in most tissues.,Eukaryotic initiation factor 4A-I

Gene ID: 1973

UniProt: P60842