Ezrin antibody (pTyr353)

Catalog No. ABIN7224914

The Rabbit Polyclonal anti-Ezrin antibody has been validated for WB and ELISA. It is suitable to detect Ezrin in samples from Human, Mouse and Rat.

Quick Overview for Ezrin antibody (pTyr353) (ABIN7224914)

Target: Ezrin (EZR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Ezrin Antibody

Binding Specificity: pTyr353

Purpose: Ezrin (phospho Tyr353) Polyclonal Antibody

Specificity: Phospho-Ezrin (Y353) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at Y353.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Ezrin Phospho-Tyr353

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background: Rabbit Anti-Ezrin (phospho Tyr353) Polyclonal Antibody,EZR, VIL2, Ezrin, Cytovillin, Villin-2, p81,The cytoplasmic peripheral membrane protein encoded by EZR (ezrin) functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for EZR. Alternatively spliced variants have also been described for EZR.,Ezrin

Molecular Weight: observerd band 69kDa

Gene ID: 7430

UniProt: P15311

Pathways: Maintenance of Protein Location