Factor 12 Heavy Chain (F12) (AA 300-380), (Cleaved-Arg372) antibody

Catalog No. ABIN7212516

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB and ELISA. Suitable for Human.

Quick Overview for Factor 12 Heavy Chain (F12) (AA 300-380), (Cleaved-Arg372) antibody (ABIN7212516)

Target: Factor 12 Heavy Chain (F12)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: AA 300-380, Cleaved-Arg372

Purpose: Cleaved-Factor XII HC (R372) Polyclonal Antibody

Specificity: Cleaved-Factor XII HC (R372) Polyclonal Antibody detects endogenous levels of fragment of activated Factor XII HC protein resulting from cleavage adjacent to R372.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Factor XII HC at AA range: 300-380

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: Factor 12 Heavy Chain (F12)

Alternative Name: Factor XII HC

Background: Rabbit Anti-Cleaved-Factor XII HC (R372) Polyclonal Antibody,F12, Coagulation factor XII, Hageman factor, HAF,F12 encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in F12 do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged.,Coagulation factor XII

Molecular Weight: observerd band 41kDa

Gene ID: 2161

UniProt: P00748