Factor Xa Activated HC (AA 190-270), (Cleaved-Ile235) antibody

Catalog No. ABIN7220410

The Rabbit Polyclonal anti-Factor Xa Activated HC antibody is suitable to detect Factor Xa Activated HC in samples from Human, Mouse and Rat. It has been validated for ELISA and WB.

Quick Overview for Factor Xa Activated HC (AA 190-270), (Cleaved-Ile235) antibody (ABIN7220410)

Target: Factor Xa Activated HC

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Binding Specificity: AA 190-270, Cleaved-Ile235

Purpose: Rabbit Anti-Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody

Specificity: Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody detects endogenous levels of fragment of activated Factor Xa activated HC protein resulting from cleavage adjacent to I235.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Factor Xa activated HC at AA range: 190-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Factor Xa Activated HC

Background: F10, Coagulation factor X, Stuart factor, Stuart-Prower factorF10 encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds, the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Molecular Weight: 30kD

Gene ID: 2159

UniProt: P00742