FZD7 antibody (AA 20-100)

Catalog No. ABIN7214956

The Rabbit Polyclonal anti-FZD7 antibody has been validated for WB, ELISA and IF. It is suitable to detect FZD7 in samples from Human and Mouse.

Quick Overview for FZD7 antibody (AA 20-100) (ABIN7214956)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-FZD7 Antibody

Binding Specificity: AA 20-100

Purpose: Frizzled-7 Polyclonal Antibody

Specificity: Frizzled-7 Polyclonal Antibody detects endogenous levels of Frizzled-7 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Frizzled-7 at AA range: 20-100

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for FZD7

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: Frizzled-7

Background: Rabbit Anti-Frizzled-7 Polyclonal Antibody,FZD7, Frizzled-7, Fz-7, hFz7, FzE3,Members of the frizzled gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. Frizzled-7 contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.,Frizzled-7

Molecular Weight: observerd band 64kDa

Gene ID: 8324

UniProt: O75084

Pathways: WNT Signaling, Stem Cell Maintenance