FZD8 antibody (AA 460-540)

Catalog No. ABIN7225246

This anti-FZD8 antibody is a Rabbit Polyclonal antibody detecting FZD8 in WB, ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for FZD8 antibody (AA 460-540) (ABIN7225246)

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FZD8 Antibody

Binding Specificity: AA 460-540

Purpose: Frizzled-8 Polyclonal Antibody

Specificity: Frizzled-8 Polyclonal Antibody detects endogenous levels of Frizzled-8 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Frizzled-8 at AA range: 460-540

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for FZD8

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Alternative Name: Frizzled-8

Background: Rabbit Anti-Frizzled-8 Polyclonal Antibody,FZD8, Frizzled-8, Fz-8, hFz8,This intronless gene FZD8 (frizzled class receptor 8) is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. FZD8 is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.,Frizzled-8

Molecular Weight: observerd band 70kDa

Gene ID: 8325

UniProt: Q9H461

Pathways: WNT Signaling