Frizzled5/8 antibody (AA 10-90)

Catalog No. ABIN7225248

This anti-Frizzled5/8 antibody is a Rabbit Polyclonal antibody detecting Frizzled5/8 in WB, ELISA and IF. Suitable for Human, Mouse and Rat.

Quick Overview for Frizzled5/8 antibody (AA 10-90) (ABIN7225248)

Target: Frizzled5/8 (HYFZD5/8)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Frizzled5/8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Frizzled5/8 Antibody

Binding Specificity: AA 10-90

Purpose: Frizzled-5/8 Polyclonal Antibody

Specificity: Frizzled-5/8 Polyclonal Antibody detects endogenous levels of Frizzled-5/8 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Frizzled-5/8 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Frizzled5/8

Target: Frizzled5/8 (HYFZD5/8)

Alternative Name: Frizzled-5/8

Background: Rabbit Anti-Frizzled-5/8 Polyclonal Antibody,FZD5, C2orf31, Frizzled-5, Fz-5, hFz5, FzE5, FZD8, Frizzled-8, Fz-8, hFz8,This intronless gene FZD8 (frizzled class receptor 8) is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. FZD8 is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.,Frizzled-5

Molecular Weight: observerd band 75kDa

Gene ID: 8325, 7855

Pathways: WNT Signaling