GPR31 antibody (AA 150-230)

Catalog No. ABIN7221238

This Rabbit Polyclonal antibody specifically detects GPR31 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for GPR31 antibody (AA 150-230) (ABIN7221238)

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GPR31 Antibody

Binding Specificity: AA 150-230

Purpose: GPR31 Polyclonal Antibody

Specificity: GPR31 Polyclonal Antibody detects endogenous levels of GPR31 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human GPR31 at AA range: 150-230

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GPR31

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Alternative Name: GPR31

Background: Rabbit Anti-GPR31 Polyclonal Antibody,GPR31, 12-(S)-hydroxy-5, 8,10,14-eicosatetraenoic acid receptor, 12-(S)-HETE receptor, 12-HETER, G-protein coupled receptor 31,GPR31 (G Protein-Coupled Receptor 31) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Peptide ligand-binding receptors. GO annotations related to this gene include G-protein coupled receptor activity. An important paralog of this gene is HCAR3. igh-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.,12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor

Molecular Weight: observerd band 33kDa

Gene ID: 2853

UniProt: O00270