GRK1 antibody (pSer21)

Catalog No. ABIN7224022

The Rabbit Polyclonal anti-GRK1 antibody (ABIN7224022) specifically detects GRK1 in WB, ELISA and IHC (p). The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for GRK1 antibody (pSer21) (ABIN7224022)

Target: GRK1 (G Protein-Coupled Receptor Kinase 1 (GRK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRK1 Antibody

Binding Specificity: pSer21

Purpose: GRK 1 (phospho Ser21) Polyclonal Antibody

Specificity: Phospho-GRK 1 (S21) Polyclonal Antibody detects endogenous levels of GRK 1 protein only when phosphorylated at S21.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human GRK 1 Phospho-Ser21

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GRK1

Target: GRK1 (G Protein-Coupled Receptor Kinase 1 (GRK1))

Alternative Name: GRK 1

Background: Rabbit Anti-GRK 1 (phospho Ser21) Polyclonal Antibody,GRK1, RHOK, Rhodopsin kinase, RK, G protein-coupled receptor kinase 1,GRK1 (G protein-coupled receptor kinase 1) encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2).,Rhodopsin kinase

Molecular Weight: observerd band 63kDa

Gene ID: 6011

UniProt: Q15835

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction