GCSc-gamma (Internal Region) antibody

Catalog No. ABIN7215036

The Rabbit Polyclonal anti-GCSc-gamma antibody is suitable to detect GCSc-gamma in samples from Human, Mouse and Rat. It has been validated for ELISA and WB.

Quick Overview for GCSc-gamma (Internal Region) antibody (ABIN7215036)

Target: GCSc-gamma

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Binding Specificity: Internal Region

Purpose: GCSc-γ Polyclonal Antibody

Specificity: GCSc-γ Polyclonal Antibody detects endogenous levels of GCSc-γ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human GCSc-gamma

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: GCSc-gamma

Background: Rabbit Anti-GCSc-γ Polyclonal Antibody,GCLC, GLCL, GLCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase,Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC encodes Glutamate--cysteine ligase catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at GCLC have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.,Glutamate--cysteine ligase catalytic subunit

Molecular Weight: observerd band 73kDa

Gene ID: 2729

UniProt: P48506