GCSm-gamma (Internal Region) antibody

Catalog No. ABIN7215038

The Rabbit Polyclonal anti-GCSm-gamma antibody (ABIN7215038) specifically detects GCSm-gamma in ELISA, WB and IHC (p). The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for GCSm-gamma (Internal Region) antibody (ABIN7215038)

Target: GCSm-gamma

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: Internal Region

Purpose: GCSm-γ Polyclonal Antibody

Specificity: GCSm-γ Polyclonal Antibody detects endogenous levels of GCSm-γ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human GCSm-gamma

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: GCSm-gamma

Background: Rabbit Anti-GCSm-γ Polyclonal Antibody,GCLM, GLCLR, Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit,Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.,Glutamate--cysteine ligase regulatory subunit

Molecular Weight: observerd band 31kDa

Gene ID: 2730

UniProt: P48507