GIT1 antibody (AA 530-610)

Catalog No. ABIN7215060

This Rabbit Polyclonal antibody specifically detects GIT1 in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for GIT1 antibody (AA 530-610) (ABIN7215060)

Target: GIT1

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GIT1 Antibody

Binding Specificity: AA 530-610

Purpose: GIT1 Polyclonal Antibody

Specificity: GIT1 Polyclonal Antibody detects endogenous levels of GIT1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human GIT1 at AA range: 530-610

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GIT1

Target: GIT1

Alternative Name: GIT1

Background: Rabbit Anti-GIT1 Polyclonal Antibody,GIT1, ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1,ARF GTPase-activating protein GIT1 is an enzyme that in humans is encoded by the GIT1 gene. GIT1 contains an ARFGAP domain, Anykrin repeats and a GRK-interacting domain. The Arf-GAP domain, which enables it to act as a GTPase activating protein (GAP) for the Arf family of GTPases, has been shown to be involved in phosphorylation and inhibition of the ADRB2. If synaptic localization of GIT1 is disturbed, then this is known to affect dendritic spine morphology and formation---this is thought to occur through the Rac1/PAK1/LIMK/CFL1 pathway.,ARF GTPase-activating protein GIT1

Molecular Weight: observerd band 95kDa

Gene ID: 28964

UniProt: Q9Y2X7