GCH1 antibody

Catalog No. ABIN7228628

The Rabbit Polyclonal anti-GCH1 antibody is suitable to detect GCH1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for GCH1 antibody (ABIN7228628)

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GCH1 Antibody

Purpose: Rabbit Anti-GCH1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of GCH1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human GCH1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GCH1

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Alternative Name: GCH1

Background: GTP cyclohydrolase 1, GTP cyclohydrolase I, GTP-CH-IGCH1 encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described, however, not all variants give rise to a functional enzyme. GCH1 (GTP Cyclohydrolase 1) is a Protein Coding gene. Diseases associated with GCH1 include Hyperphenylalaninemia, Bh4-Deficient, B and Dystonia, Dopa-Responsive, With Or Without Hyperphenylalaninemia. Among its related pathways are eNOS activation and regulation and Metabolism.

Molecular Weight: 27kD

Gene ID: 2643

UniProt: P30793