HNF4A antibody (pSer313)

Catalog No. ABIN7213334

The Rabbit Polyclonal anti-HNF4A antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect HNF4A in samples from Human, Mouse and Rat.

Quick Overview for HNF4A antibody (pSer313) (ABIN7213334)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HNF4A Antibody

Binding Specificity: pSer313

Purpose: HNF4-α (phospho Ser313) Polyclonal Antibody

Specificity: Phospho-HNF4-α (S313) Polyclonal Antibody detects endogenous levels of HNF4-α protein only when phosphorylated at S313.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human HNF4-alpha Phospho-Ser313

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4-alpha

Background: Rabbit Anti-HNF4-α (phospho Ser313) Polyclonal Antibody,HNF4A, HNF4, NR2A1, TCF14, Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4,Hepatocyte nuclear factor 4 alpha encoded by HNF4A is a nuclear transcription factor which binds DNA as a homodimer. Hepatocyte nuclear factor 4 alpha controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of HNF4A results in multiple transcript variants encoding several different isoforms.,Hepatocyte nuclear factor 4-alpha

Molecular Weight: observerd band 52kDa

Gene ID: 3172

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process