Histone 3 antibody (pThr3)

Catalog No. ABIN7225278

The Rabbit Polyclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC (p).

Quick Overview for Histone 3 antibody (pThr3) (ABIN7225278)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone 3 Antibody

Binding Specificity: pThr3

Purpose: Histone H3 (phospho Thr3) Polyclonal Antibody

Specificity: Phospho-Histone H3 (T3) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at T3.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Histone H3 Phospho-Thr3

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Rabbit Anti-Histone H3 (phospho Thr3) Polyclonal Antibody,HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H3A (histone cluster 1 H3 family member a) is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from HIST1H3A lack polyA tails, instead, they contain a palindromic termination element. HIST1H3A is found in the large histone gene cluster on chromosome 6p22-p21.3.,Histone H3.1

Molecular Weight: observerd band 20kDa

Gene ID: 8350, 8351, 8352

UniProt: P68431